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The information of the off-target loci and primer pairs used is listed in Supplementary Table S2

Semaglutide Both tirzepatide and semaglutide are powerful tools, but they act slightly differently: Semaglutide A GLP-1 receptor agonist Weekly injection (brand names Ozempic for diabetes, Wegovy for weight loss) Helps many patients achieve 1015% weight loss with lifestyle support Tirzepatide Dual-action: mimics GLP-1 and GIP (another metabolic hormone) Weekly injection (brand names Mounjaro and Zepbound) Some clinical trials show average weight loss of 1520% or more Because tirzepatide acts on two hormone pathways, it may have enhanced benefits for blood sugar control and weight reduction

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