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2.8 Spectroscopic MSA-DH or MDH activity assays The activity of MSA-DH or MDH was measured in a continuous spectroscopic assay by determining the initial rate of NADH formation at 340 nm, at a temperature of 27C using an extinction coefficient of 340 = 6.2 mM 1 cm 1 (Hossain et al., 1984)

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Step 3: Monitor your toxic load