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The red blood cells were washed three times with 500 l of 0.9% sodium chloride, after which 500 l of the erythrocyte suspension was combined with 2 ml of ice-cold double-distilled water

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In experimental applications, KLOW may be employed to investigate synergistic or additive effects among its component peptides in cell culture models, including adipocyte differentiation assays, mitochondrial respiration studies using Seahorse or Oroboros platforms, and metabolic flux analyses employing isotope tracer methodologies