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A prospective, randomised design would provide stronger evidence

Treatment for B12 deficiency can be oral, applied under the tongue or administered through the nose, or it may require various types of injections

The slides were mounted with Fluoprep (BioMerieux), and immunofluorescence was viewed and analyzed using an Imager Z1 fluorescence microscope equipped with a Digital Camera (Zeiss, Jena, Germany) connected to an AxioVs40 software (Zeiss), and with an LMS800 confocal microscope piloted by a ZEN 2.3 software (Zeiss)

A functional evaluation of anti-fatigue and exercise performance improvement following vitamin B complex supplementation in healthy humans, a randomized double-blind trial