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Insert the needle into the Melanotan I vial With the bac water in your syringe, insert the needle into the Melanotan I vial at a slight angle to avoid pressure buildup
Warren D Smith - Acquisition of data, Analysis of data, Revising the work critically for important intellectual content, Final approval of the version to be published, Agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved
Thereafter, the sequencing reaction was performed with the same primers used for amplification and Sanger sequencing was performed (ABI PRISM 3100-Avant Analyser, Applied Biosystems, Foster City, CA) in the following conditions: 1 min at 96 C and 25 cycles of 10 sec at 96 C, 5 sec at 56 C and 4 min at 68 C (with a sensitivity threshold of approximately 25%) 91
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